| Abstrakt: |
It is shown that activation of oxygen in model chemical and biological systems associated with the formation of superoxide anion radicals () leads to a decrease in the content of molecular oxygen in the buffer. The following chemical and enzymatic reactions were considered: autooxidation of adrenaline in an alkaline medium, phenazine methosulfate/NADH, as well as the xanthine–xanthine oxidase reaction. It was found that when the chemical transformation of the substrate occurs in each of these reactions O2 is consumed from the buffer, leading to temporary hypoxic conditions. Nitro blue tetrazolium and superoxide dismutase were used to determine the production of anion radicals . It was discovered for the first time that is produced in the glucose–glucose oxidase reaction, which is known as a reaction that does not generate superoxide. Catalase in reactions reacted with hydrogen peroxide, the product of disproportionation, and molecular oxygen was detected under study buffer. Thus, the following oxygen conversions in the studied reactions are shown: О2 → → Н2О2 → О2. They may also occur in intracellular systems with the involvement of appropriate oxidoreductases and antioxidant enzymes. [ABSTRACT FROM AUTHOR] |
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