Line-Shaped Illumination: A Promising Configuration for a Flexible Two-Photon Microscopy Setup.

Autor: Parravicini, Jacopo, Hasani, Elton, Tartara, Luca
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Zdroj: Applied Sciences (2076-3417); Apr2022, Vol. 12 Issue 8, p3938, 11p
Abstrakt: Featured Application: Two-photon microscopy imaging, real-time two-photon imaging, nonlinear spectral measurements, dye characterization, biological sample imaging. An innovative two-photon microscope exploiting a line-shaped illumination has been recently devised and then implemented. Such configuration allows to carry out a real-time detection by means of standard CCD cameras and is able to maintain the same resolution as commonly used point-scanning devices, thus overcoming what is usually regarded as the main limitation of line-scanning microscopes. Here, we provide an overview of the applications in which this device has been tested and has proved to be a flexible and efficient tool, namely imaging of biological samples, in-depth sample reconstruction, two-photon spectra detection, and dye cross-section measurements. These results demonstrate that the considered setup is promising for future developments in many areas of research and applications. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index