| Autor: |
He, Haiqi, Lowry, Virginia K., Swaggerty, Christina L., Ferro, Pamela J., Kogut, Michael H. |
| Předmět: |
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| Zdroj: |
FEMS Immunology & Medical Microbiology; Jan2005, Vol. 43 Issue 1, p81-89, 9p |
| Abstrakt: |
Abstract: Unmethylated CpG oligodinucleotides (CpG-ODN) flanked by specific bases found in bacterial DNA are known to stimulate innate immune responses. In this study, synthetic CpG-ODNs were evaluated for their in vitro stimulation of leukocyte and in vivo protection against Salmonella enteritidis (SE) in neonatal chickens. Our studies showed that CpG-ODN stimulated bactericidal activities, releasing granules (degranulation) and generating reactive oxygen species (oxidative burst), in chicken heterophils and up regulated nitric oxide production in chicken peripheral blood monocytes. When day-old chickens were given (i.p.) synthetic CpG-ODNs followed by oral challenge of SE, a significant reduction (p<0.05) of organ invasion by SE was observed in chickens pretreated with CpG-ODN containing the immunostimulatory GTCGTT motif. This CpG-OND also significantly reduced mortality of chickens with acute peritoneal infection of SE. Our study provides evidence that immunostimulatory CpG-ODN stimulated innate immune activities and enhanced the resistance to infectious pathogens in neonatal chickens. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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