| Abstrakt: |
Forest ecosystems are the main reservoirs of global biodiversity. Microorganisms play a key role in the biochemical cycles of these ecosystems and are used to protect plants and stimulate their growth. Due to the limitations in the application of classic methods to isolate and identify the endophytic microorganisms, their potentials and functions have yet to be properly understood. In this study, a culture-independent method was used to study the endophytic bacterial communities of some wild legumes. To this end, Zagros forests of Kermanshah were surveyed and two varieties of Astragalus ovinus and Vicia lutea were sampled. Total DNAs from different parts of the sampled plants were extracted and the bacterial 16S rDNA genes were subsequently amplified. Sequencing analysis using the Illumina MiSeq platform showed the most abundant endophytic bacteria belonged to seven orders of Rhizobiales, Xanthomonadales, Sphingomonadales, Pseudomonadales, Chitinophagales, Enterobacteriales and Betaproteobacteriales. The latter were comprised to three classes of Gammaproteobacteria, Alphaproteobacteria, Bacteroidia, and two phyla of Proteobacteri and Bacteroidetes. Our findings showed that members of Proteobacteria were the dominant bacterial endophytes and accounted for more than 99% and 68% of the total population of endophytic bacteria in V. lutea and A. ovinus, respectively. These bacteria, with the highest percentage of taxonomic units (OTUs), have the highest potential to use as biological control agents of crop legumes diseases. This study provides a clear picture of the population, dominance and diversity of bacterial endophytes of wild legumes in the Zagros forests using metagenomic analysis. [ABSTRACT FROM AUTHOR] |
