| Autor: |
Brovkina, A. S., Ushakov, R. E., Vassilieva, I. O., Domnina, A. P., Burova, E. B. |
| Zdroj: |
Cell & Tissue Biology; Apr2022, Vol. 16 Issue 2, p130-139, 10p |
| Abstrakt: |
The present work describes ability to chondrogenic differentiation of human mesenchymal stromal cells (eMSCs) derived from desquamated endometrium in menstrual blood. Neither eMSCs chondrogenic capacity nor related signaling pathways have been studied yet. eMSCs monolayer culture cultivated in chondrogenic medium within 11-14 days demonstrates chondrogenesis markers such as positive staining with Safranin O and with Alcian blue as well as the increased COL2A1 expression level. We studied linkage between expression of chondrogenic markers and activation status of PI3K and MAPK pathways by cultivating eMSCs in chondrogenic medium in the presence of PI3K inhibitor LY294002; we found that PI3K/Akt/mTOR signaling negatively regulates COL1A1 expression and positively regulates COL2A1 during differentiation and that it is also involved in regulation of Raf-MEK-ERK kinase activity. These results suggest that PI3K/Akt/mTOR pathway plays significant role in regulation of eMSCs chondrogenesis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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