Functionalized graphene oxide nanosheets with folic acid and silk fibroin as a novel nanobiocomposite for biomedical applications.

Autor: Eivazzadeh-Keihan, Reza, Alimirzaloo, Farkhondeh, Aghamirza Moghim Aliabadi, Hooman, Bahojb Noruzi, Ehsan, Akbarzadeh, Ali Reza, Maleki, Ali, Madanchi, Hamid, Mahdavi, Mohammad
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Zdroj: Scientific Reports; 4/15/2022, Vol. 12 Issue 1, p1-12, 12p
Abstrakt: In this paper, a novel graphene oxide-folic acid/silk fibroin (GO-FA/SF) nanobiocomposite scaffold was designed and fabricated using affordable and non-toxic materials. The GO was synthesized using the hummer method, covalently functionalized with FA, and then easily conjugated with extracted SF via the freeze-drying process. For characterization of the scaffold, several techniques were employed: Fourier-transform infrared (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX), and thermogravimetric analysis (TGA). The cell viability method, hemolysis, and anti-biofilm assays were performed, exploring the biological capability of the nanobiocomposite. The cell viability percentages were 96.67, 96.35 and 97.23% for 24, 48, and 72 h, respectively, and its hemolytic effect was less than 10%. In addition, it was shown that this nanobiocomposite prevents the formation of Pseudomonas aeruginosa biofilm and has antibacterial activity. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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