| Autor: |
Xu, Mengyang, Bo, Binshi, Pei, Mengchao, Chen, Yuyan, Shu, Christina Y, Qin, Qikai, Hirschler, Lydiane, Warnking, Jan M, Barbier, Emmanuel L, Wei, Zhiliang, Lu, Hanzhang, Herman, Peter, Hyder, Fahmeed, Liu, Zhi-jie, Liang, Zhifeng, Thompson, Garth J |
| Zdroj: |
Journal of Cerebral Blood Flow & Metabolism; May2022, Vol. 42 Issue 5, p811-825, 15p |
| Abstrakt: |
Functional magnetic resonance imaging (fMRI) techniques using the blood-oxygen level-dependent (BOLD) signal have shown great potential as clinical biomarkers of disease. Thus, using these techniques in preclinical rodent models is an urgent need. Calibrated fMRI is a promising technique that can provide high-resolution mapping of cerebral oxygen metabolism (CMRO2). However, calibrated fMRI is difficult to use in rodent models for several reasons: rodents are anesthetized, stimulation-induced changes are small, and gas challenges induce noisy CMRO2 predictions. We used, in mice, a relaxometry-based calibrated fMRI method which uses cerebral blood flow (CBF) and the BOLD-sensitive magnetic relaxation component, R2′, the same parameter derived in the deoxyhemoglobin-dilution model of calibrated fMRI. This method does not use any gas challenges, which we tested on mice in both awake and anesthetized states. As anesthesia induces a whole-brain change, our protocol allowed us to overcome the former limitations of rodent studies using calibrated fMRI. We revealed 1.5-2 times higher CMRO2, dependent upon brain region, in the awake state versus the anesthetized state. Our results agree with alternative measurements of whole-brain CMRO2 in the same mice and previous human anesthesia studies. The use of calibrated fMRI in rodents has much potential for preclinical fMRI. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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