| Autor: |
Srinath, Mote, Shailaja, Aayeti, Bindu, Byreddi Bhavani Venkata, Giri, Charu Chandra |
| Zdroj: |
Plant Cell, Tissue & Organ Culture; May2022, Vol. 149 Issue 1/2, p335-349, 15p |
| Abstrakt: |
Andrographis paniculata (Burm. F.) Nees is a rich source of biologically active andrographolide and other diterpene lactone compounds. The in vitro adventitious root culture system is an improved alternative strategy for mass cultivation of the plants and enhanced production of secondary metabolites in general and diterpene lactones in particular. The present study was initially envisaged, to find out how two different andrographolide biosynthetic pathways (MVA and MEP) contribute towards the accumulation of andrographolide under the influence of light. In addition, the effect of different liquid culture techniques were evaluated for enhanced biomass and elicitation facilitated andrographolide production. The culturing of adventitious roots on a platform and by not immersing directly in the liquid medium was found best for improved biomass generation. With 1.23 g of initial inoculum were able to generate 4.45 fold (5.48 g) biomass in just 4 weeks by this modified liquid culturing technique. Further, the elicitation of adventitious roots with ethrel enhanced both biomass (up to fivefold) and andrographolide content (fourfold). The differential gene expression results indicated that, under the influence of light, the MEP pathway was found to dominate for the biosynthesis of andrographolide by the up regulation of the DXR, DXS, HDR, HDS genes as well as down stream GGPS gene expression. In absence of light, MVA pathway was observed as a major contributor for the production of andrographolide. The andrographolide content was enhanced upto 4.29 fold in light elicited adventitious roots compared to the control roots (grown in the absence of light). Our results implied that the light has not only significantly enhanced the MEP pathway gene expression but also inturn elevated the andrographolide accumulation in adventitious root cultures of A. paniculate indicating the role of elicitation and tissue specific gene expression. Key message: The liquid culture technique, ethrel elicitation promoted enhanced adventitious root biomass (5 fold) and andrographolide content (4 fold). Light has shown significant effect on andrographolide production (4.29 fold) by upregulating the expression of MEP pathway and downstream genes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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