| Autor: |
Maciel, Geveraldo, Lopes, Adriana Aparecida, Cantrell, Charles L., de Castro França, Suzelei, Bertoni, Bianca Waleria, Lourenço, Miriam Verginia |
| Zdroj: |
Plant Cell, Tissue & Organ Culture; May2022, Vol. 149 Issue 1/2, p363-369, 7p |
| Abstrakt: |
Eclipta prostrata (L.) L. is widely used in traditional medicine for treatment of hepatitis, poisoning from snake bites and viral infections. Pharmacological studies confirmed its antioxidant, anti-inflammatory and anticancer activities. The efficacy of E. prostrata (L.) L. extracts has been correlated to phenylpropanoids such as flavonoids, coumestans and caffeoylquinic acid derivatives. In this work, the production of wedelolactone, demethylwedelolactone and 3,5-di-O-caffeoylquinic acid (3,5-diCQA) in hairy root cultures of E. prostrata (L.) L. C19 clone was increased after addition of eliciting agents jasmonic acid (JA) or methyl jasmonate (MeJA) at multiple concentrations. Cultures elicited with 100 μM of JA saw a 5.2 fold increase in wedelolactone (from 0.72 to 3.72 mg/g d.w.), a 1.6 fold increase in demethylwedelolactone (from 5.54 to 9.04 mg/g d.w.) and a 2.47 fold increase in 3,5-diCQA (from 18.08 to 44.71 mg/g d.w.). Obtained data validate the potential of E. prostrata (L.) L. hairy root cultures as a production system of wedelolactone, demethylwedelolactone and especially 3,5-diCQA, which has recently been reported to possess activity against coronavirus disease (Covid-19) by in silico computational studies. Key message: The goal of this work was to evaluate the effect of jasmonic acid (JA) or methyl jasmonate (MeJA) on wedelolactone, demethylwedelolactone and 3,5-di-O-caffeoylquinic acid (anti-Covid-19 drug candidate) production from hairy root cultures of Ecliptaprostrata (L.) L. C19 clone. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink