Measurement of homonuclear three-bond J(HNHα) coupling constants in unlabeled peptides complexed with labeled proteins: Application to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of hepatitis C virus (HCV).

Autor: Cicero, Daniel O., Barbato, Gaetano, Koch, Uwe, Ingallinella, Paolo, Bianchi, Elisabetta, Sambucini, Sonia, Neddermann, Petra, De Francesco, Raffaele, Pessi, Antonello, Bazzo, Renzo
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Zdroj: Journal of Biomolecular NMR; May2001, Vol. 20 Issue 1, p23-29, 7p
Abstrakt: A new isotope-filtered experiment has been designed to measure homonuclear three-bond J(HNHα) coupling constants of unlabeled peptides complexed with labeled proteins. The new experiment is based on the 3D HNHA pulse scheme, and belongs to the `quantitative J-correlation' type. It has been applied to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of human hepatitis C virus (HCV). [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index