| Autor: |
Ribeiro, Amanda Oliveira, de Oliveira, Arthur Casulli, Costa, Juliana Mara, Nachtigall, Pedro Gabriel, Herkenhoff, Marcos Edgar, Campos, Vinicius Farias, Delella, Flávia Karina, Pinhal, Danillo |
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| Zdroj: |
Developmental Dynamics; Apr2022, Vol. 251 Issue 4, p556-576, 21p |
| Abstrakt: |
MicroRNAs (miRNAs) are small noncoding RNAs with pivotal roles in the control of gene expression. By comparing the miRNA profiles of uninjured vs. regenerating tissues and structures, several studies have found that miRNAs are potentially involved in the regenerative process. By inducing miRNA overexpression or inhibition, elegant experiments have directed regenerative responses validating relevant miRNA‐to‐target interactions. The zebrafish (Danio rerio) has been the epicenter of regenerative research because of its exceptional capability to self‐repair damaged tissues and body structures. In this review, we discuss recent discoveries that have improved our understanding of the impact of gene regulation mediated by miRNAs in the context of the regeneration of fins, heart, retina, and nervous tissue in zebrafish. We compiled what is known about the miRNA control of regeneration in these tissues and investigated the links among up‐regulated and down‐regulated miRNAs, their putative or validated targets, and the regenerative process. Finally, we briefly discuss the forthcoming prospects, highlighting directions and the potential for further development of this field. Key Findings: One to several hundreds of miRNA genes have differential expression during the phases of regeneration in tissues of adult zebrafish, recapitulating the regulatory expression signatures active during early stages of development.The regulatory activity of miRNAs is temporally dependent on the stage of regeneration in which the tissue is. Different miRNAs act at different times after tissue injury, with quantitative alteration of expression according to the requirements controlled by cells active in the region of the lesion. This fine‐tuned control allows the precise adjustment in the expression level of genes and their protein products required at each stage of regeneration.In the major groups of vertebrates, during the regeneration of limbs, organs and structures, the regulatory program mediated by microRNAs is remarkably more conserved at the intratissue level between different species than at the inter‐tissue level within a single individual of any given species.Broad comparative analyses of microRNA transcriptome, especially by comprehensive datasets generated by RNA‐seq, are informative about the scenario of molecules with a central role in post‐injury regeneration among zebrafish tissues and represent a valuable strategy for identifying targets for studies in humans. A notable example is miR‐21, which is a uniquely differentially expressed miRNA in multiple zebrafish tissues during regeneration and whose mature transcripts are evolutionarily conserved in human cells, providing new perspectives for translational research.Given the versatility of the zebrafish model and its use as an animal model for structural and functional genome research and the control in the use of new techniques such as genome editing by CRISPR, we believe that the investigation of the role of miRNAs in regeneration will be rapidly increased. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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