| Autor: |
Nor, Zairossani M., Tamer, Melih I., Mehrvar, Mehrab, Scharer, Jeno M., Moo-Young, Murray, Jervis, Eric J. |
| Předmět: |
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| Zdroj: |
Biotechnology Letters; Jun2001, Vol. 23 Issue 11, p845-849, 5p |
| Abstrakt: |
Four fed-batch control strategies were evaluated to improve the specific lactase activity of Kluyveromyces fragilis. Control strategies tested included DO-stat control, exponential feeding, exponential feeding with manual feedback control and corrected feed-forward control. Each was implemented with standard sensors (i.e., temperature, dissolved oxygen and pH sensors) commonly installed in fermenters. The highest specific activity was obtained using the corrected feed-forward control strategy, a strategy incorporating a novel method for on-line estimation of specific growth rate. The control strategy was able to operate effectively to a final cell density of 69 g dry wt l-1 with a specific lactase activity of 2 U mg-1 cell dry wt. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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