New expanded bed adsorbents for the recovery of DNA.

Autor: Theodossiou, Irini, Olander, Morten A., Søndergaard, Michael, Thomas, Owen R. T.
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Zdroj: Biotechnology Letters; Dec2000, Vol. 22 Issue 24, p1929-1933, 5p
Abstrakt: A 20–40 μm pellicular high density (∼3.7 g cm-3) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml-1, c.f. ∼50 μg ml-1 at 10% breakthrough), due to a combination of small particle and `fuzzy' surface architecture created through the coupling of polyethylene imine chains. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index