| Autor: |
Taiwo, Kehinde M., Olenginski, Lukasz T., Nußbaumer, Felix, Nam, Hyeyeon, Hilber, Stefan, Kreutz, Christoph, Dayie, T. Kwaku |
| Zdroj: |
Chemical Monthly / Monatshefte für Chemie; Mar2022, Vol. 153 Issue 3, p293-299, 7p |
| Abstrakt: |
Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-15N]-guanosine-5′-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink