| Abstrakt: |
This study assessed the genotoxic sensitivity of cotton bollworm, Pectinophora gossypiella (Saund.) larvae treated at a 1-day-old egg stage with azadirachtin and Fertacho exposed to gamma rays (400 and 700 Gy). Comet assay or genotoxicity assessments were based on length, height, area, and intensity of head or tail for the comet parameters. Head diameter, DNA in the head and tail (%), and tail moment were affected by both azadirachtin and Fertacho, leading to the death of most cells, DNA degradation, or increased cell division and subsequent malformation of cells compared to normal P. gossypiella larvae. In addition, DNA had destruction grades according to treatments ranging from 0 to 1. Moreover, the resistant cells of P. gossypiella were investigated by fluorescence microscopy, which showed cell deformities, loss of cell wall, and swelling or shrinking of the cells. Fertacho exposed at 400 Gy was a highly genotoxic compound compared to azadirachtin exposed at 700 Gy. [ABSTRACT FROM AUTHOR] |
