| Autor: |
Zhang, Yali, Wei, Min, Wu, Yangtao, Wang, Juan, Hong, Yuting, Huang, Yang, Yuan, Lunzhi, Ma, Jian, Wang, Kai, Wang, Shaojuan, Shi, Yang, Wang, Zikang, Guo, Huilin, Xiao, Jin, Yang, Chuanlai, Ye, Jianghui, Chen, Jijing, Liu, Yuxi, Fu, Baorong, Lan, Miaolin |
| Zdroj: |
Cell Reports; Mar2022, Vol. 38 Issue 12, pN.PAG-N.PAG, 1p |
| Abstrakt: |
Mutations in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike receptor-binding domain (RBD) may alter viral host tropism and affect the activities of neutralizing antibodies. Here, we investigated 153 RBD mutants and 11 globally circulating variants of concern (VOCs) and variants of interest (VOIs) (including Omicron) for their antigenic changes and cross-species tropism in cells expressing 18 ACE2 orthologs. Several RBD mutations strengthened viral infectivity in cells expressing ACE2 orthologs of non-human animals, particularly those less susceptible to the ancestral strain. The mutations surrounding amino acids (aas) 439–448 and aa 484 are more likely to cause neutralization resistance. Strikingly, enhanced cross-species infection potential in the mouse and ferret, instead of the neutralization-escape scores of the mutations, account for the positive correlation with the cumulative prevalence of mutations in humans. These findings present insights for potential drivers of circulating SARS-CoV-2 variants and provide informative parameters for tracking and forecasting spreading mutations. [Display omitted] • Infection in cells with ferret or mouse ACE2 correlates with prevalence of RBD mutants • Mutations near aas 439–448 and 484 are more likely to cause neutralization resistance • Many spike variants of VOC and VOI strains show increased cross-species infection potential Zhang et al. show in vitro cross-species infectivity and neutralization-escape characteristics of 153 SARS-CoV-2 RBD mutants and 11 globally circulating VOCs and VOIs. They reveal an association between enhanced cross-species infection potential and the current cumulative prevalence of mutations, which can inform surveillance and forecasting of SARS-CoV-2 spike mutations. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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