| Autor: |
Ruiz Tejada Segura, Mayra L., Abou Moussa, Eman, Garabello, Elisa, Nakahara, Thiago S., Makhlouf, Melanie, Mathew, Lisa S., Wang, Li, Valle, Filippo, Huang, Susie S.Y., Mainland, Joel D., Caselle, Michele, Osella, Matteo, Lorenz, Stephan, Reisert, Johannes, Logan, Darren W., Malnic, Bettina, Scialdone, Antonio, Saraiva, Luis R. |
| Zdroj: |
Cell Reports; Mar2022, Vol. 38 Issue 12, pN.PAG-N.PAG, 1p |
| Abstrakt: |
The sense of smell helps us navigate the environment, but its molecular architecture and underlying logic remain understudied. The spatial location of odorant receptor genes (Olfrs) in the nose is thought to be independent of the structural diversity of the odorants they detect. Using spatial transcriptomics, we create a genome-wide 3D atlas of the mouse olfactory mucosa (OM). Topographic maps of genes differentially expressed in space reveal that both Olfrs and non- Olfrs are distributed in a continuous and overlapping fashion over at least five broad zones in the OM. The spatial locations of Olfrs correlate with the mucus solubility of the odorants they recognize, providing direct evidence for the chromatographic theory of olfaction. This resource resolves the molecular architecture of the mouse OM and will inform future studies on mechanisms underlying Olfr gene choice, axonal pathfinding, patterning of the nervous system, and basic logic for the peripheral representation of smell. [Display omitted] • We generate a browsable 3D transcriptomic atlas of the mouse olfactory mucosa (OM) • We identify potential functional hotspots in the mouse OM • Odorant receptor genes (Olfrs) are continuously distributed over at least five zones • Spatial locations of Olfrs correlate with the mucus solubility of their ligands Ruiz Tejada Segura et al. employ a spatial transcriptomics approach to create a 3D map of gene expression of the mouse nose and combine it with single-cell RNA-seq, machine learning, and chemoinformatics to resolve its molecular architecture and shed light into the anatomical logic of smell. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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