| Autor: |
Mustapha, Abdul- Rahman, Ghosh, Subrata Kumar, Prasad, Jai Kishan, Katiyar, Rahul, Kumar, Abhishek, Amin, Beigh Y., Bag, Sadhan, Bhure, Sanjeev K., Sharma, Guttula Taru, Verma, Med Ram |
| Předmět: |
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| Zdroj: |
Andrologia; Mar2022, Vol. 54 Issue 2, p1-10, 10p |
| Abstrakt: |
The present study was undertaken to determine the efficacy of partial deoxygenation of extender at constant temperature (35°C) in freezability of crossbred bull semen. The dissolved oxygen (DO) levels were reduced by the use of newly developed technique of nitrogen effervescence at a flow rate of 2-- 3 bubbles per second. Four different levels of oxygen in semen extender, that is 11.7, 2, 4 and 8 ppm as control (Group- I), Group- II, Group- III and Group- IV, respectively, were used to assess the effect of partial deoxygenation on semen quality parameters. The 4 ppm level of DO resulted in higher (p < 0.05) progressive motility in comparison with non- treated group at post- thaw stage, whereas reduction up to 2 ppm resulted in drastic fall in motility. Oxidative stress status revealed low superoxide dismutase (SOD) and total antioxidant capacity (TAC) in Group- II, whereas higher (p < 0.05) SOD and TAC activities were observed in Group- III in comparison with non- treated group at pre- freeze and post- thaw stages. The sperm- zona binding at 4 ppm level of DO was significantly higher than control group, 2 and 8 ppm levels of DO. In conclusion, reduction of DO in the extender up to 4 ppm reduced oxidative stress and improved in vitro fertility of crossbred bull spermatozoa. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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