| Autor: |
DENG Ying, LI Qiang, YAN Liheng, ZHANG Baiyu, YANG Hu, FU Ping, SUN Shanshan, YAN Qigui |
| Předmět: |
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| Zdroj: |
Journal of Northwest A & F University - Natural Science Edition; 2022, Vol. 50 Issue 1, p10-18, 9p |
| Abstrakt: |
[Objective] This study compared virulence expression of two Pseudomonas aeruginosa strains of ZP-1 (non-producing pyocyanin) and BX-1 (producing pyocyanin) from lungs of forest musk deer and their pathogenicity in acute and chronic lung infections in mice to provide basis for treatment, prevention and control of purulent pneumonia in forest musk deer. [Method] The differences in virulence expression of ZP-1 and BX-1 strains were compared and mice acute and chronic lung infection models were established using non-invasive tracheal intubation and preparation of chronic infection-embedded strains. The number of residual Pseudomonas aeruginosa, total number of white blood cells, and level of cytokines in mice lungs with acute and chronic infections were detected and histopathological changes were observed. [Result] Expression of pyoverdine and extracellular polysaccharide of ZP-1 in vitro was significantly higher than that of BX-1 (P<0.05). The extracellular proteases (LasA and LasB) activities of BX-1 strain were higher than those of ZP-1 without significant differences (P>0.05). The expression of rhatnnolipid in BX-1 was significantly higher than that in ZP-1 (P<0.05). The structure of lipopolysaccharides between ZP-1 and BX-1 was different, especially for core polysaccharides. At 12 hours of acute lung infection in mice, the residual number of Pseudomonas aeruginosa, total number of white blood cells and cytokine levels in lung tissues of the ZP-1 group were higher than those of the BX-1 group. At 24 h of infection, the levels of IL-1β and IL-6 in the BX-1 group were higher than those in the ZP-1 group. With the prolongation of chronic lung infection,the residual number of Pseudomonas aeruginosa, total number of white blood cells and content of IFN-γ in lung tissues of the ZP-1 and BX-1 groups gradually decreased, while contents of IL-1β and IL-6 gradually increased. The total number of white blood cells in the ZP-1 group was higher than that in the BX-1 group except for the third days after infection. The levels of IL-1βand IL-6 in lung tissues of the ZP-1 group were higher than those in the BX-1 group except for the 14th day after infection. There was no significant difference in IFN-γ content in lung tissues among the two strains. [Conclusion] The ZP-1 strain of Pseudomonas aeruginosa from lung of forest musk deer was more pathogenic to mice with lung infection. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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