| Autor: |
Siddiqui, Ruqaiyyah, Shah, Rida Arshad, Akbar, Noor, Khamis, Mustafa, Ibrahim, Taleb, Abouleish, Mohamed Yehia, Khan, Naveed Ahmed |
| Zdroj: |
International Ophthalmology; Mar2022, Vol. 42 Issue 3, p939-944, 6p |
| Abstrakt: |
Aim: In this study, we utilized a micelle–clay complex composed of the surfactant octadecyltrimethylammonium bromide and montmorillonite clay and evaluated its antibacterial effects. Methods: Using Pseudomonas aeruginosa, Staphylococcus epidermidis, and Micrococcus luteus, bactericidal assays were performed to determine the effects of ODTMA-clay complex on the viability of bacterial pathogen at various doses and different intervals of time. Cytotoxicity assays were performed to investigate ODTMA-clay complex effects on human cells, as determined by release of intracellular lactate dehydrogenase. Results: The results revealed that ODTMA-clay complex abolished bacterial viability at 100 μg/mL within 45 min against P. aeruginosa, S. epidermidis, and M. luteus. Cytotoxicity assays revealed that ODTMA-clay complex exhibited minimal toxicity of the human cells. Conclusion: Rapid and potent antibacterial effects of ODTMA micelle–clay complex were observed in vitro; however, research is needed to determine precise formulation of contact lens disinfectants comprising ODTMA micelle–clay complex. Additionally, studies should be conducted using in vivo models of keratitis, progressing to pre-clinical and clinical trials. ODTMA micelle–clay complex is an ideal candidate to be incorporated in a novel contact lens disinfectant given the cost-effectiveness and ease of application and can be incorporated as an effective preventative strategy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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