| Autor: |
Ivanov, Yuri D., Malsagova, Kristina A., Goldaeva, Kristina V., Pleshakova, Tatyana O., Shumov, Ivan D., Galiullin, Rafael A., Kapustina, Svetlana I., Iourov, Ivan Y., Vorsanova, Svetlana G., Ryabtsev, Stepan V., Popov, Vladimir P., Archakov, Alexander I. |
| Předmět: |
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| Zdroj: |
Genes; Feb2022, Vol. 13 Issue 2, p199, 1p |
| Abstrakt: |
MicroRNAs (miRNAs), which represent short (20 to 22 nt) non-coding RNAs, were found to play a direct role in the development of autism in children. Herein, a highly sensitive "silicon-on-insulator"-based nanosensor (SOI-NS) has been developed for the revelation of autism-associated miRNAs. This SOI-NS comprises an array of nanowire sensor structures fabricated by complementary metal–oxide–semiconductor (CMOS)-compatible technology, gas-phase etching, and nanolithography. In our experiments described herein, we demonstrate the revelation of ASD-associated miRNAs in human plasma with the SOI-NS, whose sensor elements were sensitized with oligonucleotide probes. In order to determine the concentration sensitivity of the SOI-NS, experiments on the detection of synthetic DNA analogues of autism-associated miRNAs in purified buffer were performed. The lower limit of miRNA detection attained in our experiments amounted to 10−17 M. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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