| Autor: |
Kumar, Anil, Bera, Smritilekha, Singh, Man, Mondal, Dhananjoy |
| Zdroj: |
SILICON (1876990X); Feb2022, Vol. 14 Issue 4, p1407-1419, 13p |
| Abstrakt: |
Surface functionalization of silica nanoparticles (SiNPs) with biomolecules, such as amino acids, amino sugars and carbohydrates was performed to produce surface-functionalized silica nanoparticles (f-SiNPs) through physical adsorption on the surface of colloidal SiNPs. The structural and physicochemical properties of the SiNPs and f-SiNPs were determined using SEM, FTIR, UV, DLS and NMR spectroscopy. The size of the functionalized-silica nanoparticles is found in the range of ~28.0 to 141.0 nm while the surface charge of the nanoparticles was observed to be negative except for f-SiNPs-Met, f-SiNPs-Glu and f-SiNPs-Gly. The SEM images of surface morphology of f-SiNPs described the differences from the SEM images of SiNPs that corroborated the silica nanoparticles' functionalization. The synthesized SiNPs and f-SiNPs with biomolecules have been used for interaction with plant DNA isolated from the Bixa orellana L. plant. DNA interaction was quantified using agarose gel electrophoresis, visualized with a marker, and the purity was examined by the absorption ratio of A260: A280 (1.81) of isolated DNA and the quantity of the estimated dsDNA was found to be 1495 μg/mL. Interaction of DNA with alanine-SiNPs have shown greater intercalation properties due to its hydrophobic nature that protected the action of DNA molecules, whereas lysine-encapsulated nanoparticles have revealed DNA de-naturalization or replication. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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