| Autor: |
Demin, A. M., Kandarakov, O. F., Belyavsky, A. V., Krasnov, V. P. |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2022, Vol. 2390 Issue 1, p1-5, 5p |
| Abstrakt: |
The efficiency of L-Lys-modification of Fe3O4−based magnetic nanoparticles obtained by precipitation from aqueous solutions (∼10 nm) and by the gas-phase method (∼30 nm) was investigated. It has been demonstrated that chemically obtained nanoparticles possessed more pronounced hydrophilic properties of the surface, which explains the possibility of obtaining stable aqueous colloidal solutions of nanoconjugates based thereon, as well as their somewhat more efficient modification by 3-aminopropylsilane and then L-Lys (0.25 versus 0.21 mmol/g). However, it has been found that under the labelling conditions used, the proportion of NIH 3T3 cells containing L-Lys-modified particles was greater for the sample based on gas-condensation obtained MNPs as compared to nanoconjugates based on chemically prepared MNPs. In addition, cells retained MNPs noticeably longer. So, up to 45% of the loaded MNPs remained in the cells for 3 days. The results obtained in this work can be used in the design of new nanomaterials for magnetic cell-labelling. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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