| Autor: |
Vermeer, B. J., Kardaun, S. H., Koerten, H. K., Claas, F. H. J., Ploem, J. S. |
| Předmět: |
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| Zdroj: |
British Journal of Dermatology; Jul1985 Supplement 28, Vol. 113, p118-123, 6p |
| Abstrakt: |
Incubation of skin explant culture with HLA-A, -B and -C alloantibodies results in detachment of cells at the periphery of the outgrowth. This detachment can be observed within i ] h, using the reflection contrast microscope. During the detachment of the epithelial cell, characteristic changes occur, such as disorganization of the filopodia and lifting up of the cell borders from the surface of the Petri dish. The same changes were seen using antibodies against pemphigus antigens. Antibody reactions directed against membrane-associated antigens can induce a detachment of keratinocytes from each other and from the substratum (Farb, Dykes & Lazarus, 1978; Woo et al., 1983). The same phenomenon can also be induced by antibody reactions directed against melanoma cells (Singer et al., 1982). Various studies have shown that the release of proteinase by these antibody reactions plays a crucial role in cellular detachment (Hashimoto et al., 1983). In most studies detachment was determined when the cell cultures were incubated for several hours with the antibody (Woo et al., 1983). The aim of this study was to develop a model in which changes characteristic for detachment of epithelial cells could be observed earlier. Moreover, the effect of anti-HLA-A, -B and -C alloantibodies on the epithelial cells was investigated. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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