| Autor: |
Li, Xiaoyong, Yao, Xiaoying, Mu, Chunlan, Wang, Zhaoxia, Hu, Rong, Chang, Yan, Wu, Ji |
| Předmět: |
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| Zdroj: |
Stem Cell Reviews & Reports; Jan2022, Vol. 18 Issue 1, p336-345, 10p |
| Abstrakt: |
Female germline stem cells (FGSCs) have been found in mouse, rat, pig, sheep and human ovaries. However, there is no information on the isolation or long-term culture of FGSCs from non-human primates. Here, we identified the presence of FGSCs in the ovaries of juvenile (3–4-year-old) cynomolgus monkeys using DDX4 and Ki67 double immunofluorescence. Then, a long-term serum- and cell feeder-free culture system for these FGSCs was used to establish a cell line, and its biological characteristics were analyzed. We found that testosterone promoted self-renewal of the cells. This study confirmed for the first time the presence of FGSCs in the ovary of non-human primates. This culture system and cell line will be of great significance for research in medicine and reproductive biology. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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