| Autor: |
Fang, Bin, Zhang, Beilin, Zhai, Rongxiu, Wang, Limin, Ding, Yang, Li, Huizi, Bai, Hua, Wang, Zhenhua, Peng, Bo, Li, Lin, Fu, Li |
| Předmět: |
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| Zdroj: |
New Journal of Chemistry; 2/7/2022, Vol. 46 Issue 5, p2487-2494, 8p |
| Abstrakt: |
Rational design of mitochondrial viscosity probes with water-soluble two-photon (TP) fluorescence probes still remains a challenge in practical biological applications. Herein, three novel water-soluble TP active sulfosalt derivatives (Mito-Z, Mito-H, and Mito-Q) were developed with high sensitivity to mitochondrial viscosity in living cells. In non-viscous media, the probes possess a small fluorescence brightness (εΦ) and TP action cross-sections (Φδ). With the increasing viscosity of the micro-environment, εΦ and Φδ of the probes increase significantly. Moreover, the results of TP fluorescence imaging demonstrated that Mito-Q was sensitive to local viscosity and selectively staining mitochondria. In a nutshell, Mito-Q possessed a number of beneficial properties in bioimaging such as good membrane permeability, long wavelength red emission, moderate Φδ value, high sensitivity viscosity response, and specific mitochondrial targeting, affording a promising platform for detecting mitochondrial viscosity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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