Reactivity of γ‐glutamyl‐cysteine with intracellular and extracellular glutathione metabolic enzymes.

Autor:	Muraoka, Misa, Yoshida, Saki, Ohno, Moeka, Matsuura, Hideyuki, Nagano, Kazuya, Hirata, Yoshihiko, Arai, Masayoshi, Hirata, Kazumasa
Předmět:	GLUTATHIONE FOOD additives ENZYMES GLUTATHIONE reductase GLUTATHIONE peroxidase GLUTATHIONE transferase GAMMA-glutamyltransferase
Zdroj:	FEBS Letters; Jan2022, Vol. 596 Issue 2, p180-188, 9p
Abstrakt:	Gamma‐glutamyl‐cysteine (γ‐EC) is a precursor of glutathione (GSH) biosynthesis. We investigated whether it functions as a substrate for three intracellular and one extracellular GSH metabolic enzymes, which mediate the antioxidant defence function of GSH. Among them, glutathione peroxidase, glutathione S‐transferase and γ‐glutamyl transferase (GGT) exhibited substrate specificity for γ‐EC, whereas glutathione reductase did not. The specificities of γ‐EC and its disulphide form to GGT were comparable to GSH and its oxidized form, GSSG respectively. These results indicate that they can supply GSH constituent amino acids, glutamate, cysteine and cystine through degradation by GGT. γ‐EC may contribute valuable antioxidant defence properties as a food and cosmetic additive. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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