Survival rate, blood feeding habits and sibling species composition of Aedes simpsoni complex: Implications for arbovirus transmission risk in East Africa.

Autor: Kamau, Winnie W., Sang, Rosemary, Ogola, Edwin O., Rotich, Gilbert, Getugi, Caroline, Agha, Sheila B., Menza, Nelson, Torto, Baldwyn, Tchouassi, David P.
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Zdroj: PLoS Neglected Tropical Diseases; 1/24/2022, Vol. 16 Issue 1, p1-12, 12p
Abstrakt: Aedes simpsoni complex has a wide distribution in Africa and comprises at least three described sub-species including the yellow fever virus (YFV) vector Ae. bromeliae. To date, the distribution and relative contributions of the sub-species and/or subpopulations including bionomic characteristics in relation to YF transmission dynamics remain poorly studied. In this study conducted in two areas with divergent ecosystems: peri-urban (coastal Rabai) and rural (Rift Valley Kerio Valley) in Kenya, survival rate was estimated by parity in Ae. simpsoni s.l. mosquitoes sampled using CO2-baited BG Sentinel traps. We then applied PCR targeting the nuclear internal transcribed spacer 2 (ITS2), region followed by sequencing and phylogenetic analytics to identify the sibling species in the Ae. simpsoni complex among parous and blood fed cohorts. Our results show that Ae. bromeliae was the most dominant sub-species in both areas, exhibiting high survival rates, human blood-feeding, and potentially, high vectorial capacity for pathogen transmission. We document for the first time the presence of Ae. lilii in Kenya and potentially yet-to-be described species in the complex displaying human feeding tendencies. We also infer a wide host feeding range on rodents, reptile, and domestic livestock besides humans especially for Ae. bromeliae. This feeding trend could likely expose humans to various zoonotic pathogens. Taken together, we highlight the utility of genotype-based analyses to generate precision surveillance data of vector populations for enhanced disease risk prediction and to guide cost-effective interventions (e.g. YF vaccinations). Author summary: Yellow fever (YF) remains a significant public health risk in East Africa, however, with gaps in the transmission ecology. Important YF virus vectors include Aedes simpsoni mosquitoes that comprise subspecies with varying vectoring abilities and, poorly described ecology and biologic traits relevant to disease transmission. Through active surveillance, we analyzed the survival, human blood feeding habits and genetics of wild populations of Aedes simpsoni s.l. in two contrasting ecosystems in Kenya: peri-urban, coastal Rabai, and rural, Rift Valley Kerio Valley. Our findings reveal i) Aedes bromeliae as the most abundant subspecies in both areas exhibiting high survival rates, human blood-feeding, and potentially, high vectoring ability, ii) occurrence of Ae. lilii contrary to previous reports albeit in low numbers, iii) potential undescribed species in the group displaying human feeding tendencies. Knowledge of the locally adapted subspecies and associated traits that underlie vectorial capacity, impinges on YF distribution risk useful for guiding vector control or cost-effective vaccination. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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