| Autor: |
Taqi, Muhammad, Erum, Shazia, Rasheed, Shamaila, Siddiqui, Sadar Uddin, Jatoi, Shakeel Ahmad |
| Předmět: |
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| Zdroj: |
Pakistan Journal of Agricultural Research; Dec2021, Vol. 34 Issue 4, p868-877, 10p |
| Abstrakt: |
Overharvesting has posed pressure on Caralluma tuberculata plants resulting in the disappearance of its wild populations in different mountainous regions in Pakistan. A proficient way for large-scale and fast propagation of C. tuberculata through in-vitro organogenesis was carried out at in-vitro lab, Bio-resources Conservation Institute, NARC, Islamabad, Pakistan. Nodal-tips as explants were grown on MS salts enriched with diverse plant growth regulators including BAP, NAA, Kin and 2,4-D, alone as well as in combinations. The highest growth response for callusing 78.3% was observed in BAP (0.5mg/L) in combination with Kinetin (0.25mg/L) +NAA (2 mg/L) followed by 65% at 2 mg/L of 2,4-D. Maximum percentage of shooting (81.67%) with the maximum mean value for number of shoots (7.56) and shoot length (7.07) occurred when the fresh shoot tip of C. tuberculata were planted on to MS salts containing 2 mg/L of BAP, 0.5 mg/L of NAA and 0.25 mg/L of Kin. The new shoots were then inoculated on 1/2 strength MS salts fortified with NAA and IBA root induction. The highest rooting percentage (61.67%), as well as root length (3.4 cm) was obtained with 0.5mg/L of both IBA and NAA. The current study could be beneficial for providing the basis to restore wild populations of C. tuberculata using in-vitro raised mass propagated plants, and help conserve the endangered germs of hills. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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