| Autor: |
Yang, Renyuan, Fonder, Catherine, Sylvester, Talia, Peng, Stefan, Jiles, David, Sakaguchi, Donald S., Que, Long |
| Předmět: |
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| Zdroj: |
Journal of Microelectromechanical Systems; Feb2022, Vol. 31 Issue 1, p37-44, 8p |
| Abstrakt: |
Adult hippocampal neural stem/progenitor cells (AHPCs), which are self-renewing multipotent progenitors that can differentiate into neurons, astrocytes, and oligodendrocytes, are suitable as a central nervous system (CNS) molecular model, and the formation of 3D AHPC neurospheroids are potentially suitable as an in vitro brain model. In this paper we report a new microfluidic chip to culture AHPC neurospheroids (NSs-AHPC) inside culture-chambers on chip. After cell fixation and immunostaining were conducted, the fluorescence images of NSs-AHPC were analyzed. It has been found that the AHPCs comprising neurospheroids remained highly viable. Cell proliferation and neuronal differentiation have also been observed, indicating the feasibility of NSs-AHPC as the in vitro brain model on chip. Given its simple-to-use, low-cost, and orderly arranged culture-chambers, this type of chip is particularly suitable for culturing and analyzing multiple in vitro brain models in an efficient manner. [2021-0199] [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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