Detection of in-frame mutation by IS30-family insertion sequence in the phospholipid phosphatidylglycerol synthase gene (pgsA2) of high-level daptomycin-resistant Corynebacterium striatum.

Autor:	Gotoh, Kazuyoshi, Mayura, I. Putu Bayu, Enomoto, Yusaku, Iio, Koji, Matsushita, Osamu, Otsuka, Fumio, Hagiya, Hideharu
Předmět:	INSERTION mutation PHOSPHATIDYLGLYCEROL CORYNEBACTERIUM GENE silencing MYCOSIS fungoides ECHINOCANDINS
Zdroj:	European Journal of Clinical Microbiology & Infectious Diseases; Feb2022, Vol. 41 Issue 2, p331-333, 3p
Abstrakt:	The emergence of high-level daptomycin (DAP)-resistant (HLDR) Corynebacterium striatum has been reported as a result of loss-of-function point mutations or premature stop codon mutations in a responsible gene, pgsA2. We herein describe the novel detection of an HLDR C. striatum clinical isolate, in which IS30-insertion was corroborated to cause destruction of pgsA2 gene. We isolated an HLDR C. striatum from a critically ill patient with underlying mycosis fungoides who had been treated with DAP for 10 days. With a sequence investigation, IS30-insertion was discovered to split pgsA2 in the HLDR C. striatum strain, which may cause disrupted phospholipid phosphatidylglycerol (PG) production. Future studies should survey the prevalence of IS-mediated gene inactivation among HLDR C. striatum clinical isolates. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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