| Abstrakt: |
Currently, we are dealing with ever‐increasing pollution of the environment with metal and metal oxide nanoparticles. One type of these, zinc oxide nanoparticles (ZnO‐NPs), are increasingly used in areas such as cosmetology, electrical engineering, medicine, and even in the food and textile industries. As a consequence, ZnO‐NPs may enter the human body in many ways. Their influence on the body is still not clear. Here, we define the mechanism of the initial toxicity of ZnO‐NPs to cells based on interaction with the lipid part of the native and model cell membrane. The selected cell lines react differently to contact with nanoparticles. We found a disruption of the native membranes of B16‐F0 cells and to a lesser extent of COLO 679. In turn, the membrane of COLO 679 cells was more peroxidated, and cell viability was much lower. A model of the lipid part of the membrane was created for B16‐F0 cells and compared with previously published studies on immune cells. On the basis of physicochemical parameters obtained for individual lipids and a mix representing the native membrane of the tested cells, we concluded that exposure to nanoparticles resulted in a change within the model membranes (specifically with the polar parts of lipids). The greatest interaction has been noticed between ZnO‐NPs and zwitterionic phospholipids (PC and PE), cholesterol, and negatively charged phosphatidylglycerol. Assessing the interactions between the membrane and nanoparticles will help to better understand the first steps of its toxicity mechanism. ZnO‐NPs are ever‐increasingly present in the environment and everyday products. The assessment of their safety is important in pursuing more conscious use. The study investigates the interaction between ZnO‐NPs and the cell membrane as the first structure in contact with nanoparticles. We show that exposure to nanoparticles resulted in a change within the membrane lipid polar parts. Knowledge of the stages of toxicity will contribute to determining the exact mechanism and relationship between the cell type and resistance to xenobiotics. [ABSTRACT FROM AUTHOR] |
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