A Method for Determining Characteristic Parameters of DNA Melting in Nucleic Acid Analyzers.

Autor:	Kurochkin, V. E., Belov, D. A., Belov, Yu. V., Zubik, A. N.
Předmět:	NUCLEIC acid analysis BIOCHEMISTRY DNA TIME PHENOMENOLOGY DESCRIPTIVE statistics POLYMERASE chain reaction
Zdroj:	Biomedical Engineering; Jan2022, Vol. 55 Issue 5, p333-336, 4p
Abstrakt:	Polymerase chain reaction (PCR) and DNA melting methods are run on nucleic acid analyzers. We propose a method for recording melting signals using analyzers of the ANK series that decreases errors in determining the main parameter – the DNA melting temperature Tm. We propose a method for assessing the width of the melting interval ΔT as an additional specificity criterion for PCR products. Measurement errors have been evaluated: standard deviation (RMS) for Tm, ≤0.11°C; for ΔT, ≤0.06°C. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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