The PML-RARA fusion is not detectable in historical blood samples of acute promyelocytic leukaemia patients.

Autor: Dunn, William G., Gu, Muxin S., Fabre, Margarete A., Cooper, Jonathan, Nomdedeu, Josep F., Koumas, Laura, Nicolaou, Katerina, Chi, Jiangxiang, Costeas, Paul, Vassiliou, George S.
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Zdroj: Annals of Hematology; Feb2022, Vol. 101 Issue 2, p443-445, 3p
Abstrakt: Dear Editor, Acute promyelocytic leukaemia (APL) is characterized by the presence of the t(15;17) chromosomal translocation that generates the I PML-RARA i fusion gene [[1]]. To determine the sensitivity for detecting each breakpoint by PCR, leukaemic DNA samples were suspended at 20 ng/ L and iteratively diluted tenfold to a dilution of 1:10,000 into equimolar (20 ng/ L) pooled normal blood DNA from 10 healthy subjects. Here, to investigate whether I PML-RARA i is detectable in the blood before APL onset, we study four APL cases with blood DNA available, from the same individual, 2-12 years before diagnosis. [Extracted from the article]
Databáze: Complementary Index