B1‐type cyclins control microtubule organization during cell division in Arabidopsis.

Autor: Romeiro Motta, Mariana, Zhao, Xin'Ai, Pastuglia, Martine, Belcram, Katia, Roodbarkelari, Farshad, Komaki, Maki, Harashima, Hirofumi, Komaki, Shinichiro, Kumar, Manoj, Bulankova, Petra, Heese, Maren, Riha, Karel, Bouchez, David, Schnittger, Arp
Zdroj: EMBO Reports; 1/5/2022, Vol. 23 Issue 1, p1-18, 18p
Abstrakt: Flowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitotic cyclins in cell cycle control during Arabidopsis development. While we reveal CYCB1;5 to be a pseudogene, the remaining four members were found to be expressed in dividing cells. Mutant analyses showed a complex pattern of overlapping, development‐specific requirements of B1‐type cyclins with CYCB1;2 playing a central role. The double mutant cycb1;1 cycb1;2 is severely compromised in growth, yet viable beyond the seedling stage, hence representing a unique opportunity to study the function of B1‐type cyclin activity at the organismic level. Immunolocalization of microtubules in cycb1;1 cycb1;2 and treating mutants with the microtubule drug oryzalin revealed a key role of B1‐type cyclins in orchestrating mitotic microtubule networks. Subsequently, we identified the GAMMA‐TUBULIN COMPLEX PROTEIN 3‐INTERACTING PROTEIN 1 (GIP1/MOZART) as an in vitro substrate of B1‐type cyclin complexes and further genetic analyses support a potential role in the regulation of GIP1 by CYCB1s. Synopsis: Studying multiple mutant combinations, this study reveals the role of B1‐type cyclins in plant development and microtubule organization. The B1‐type cyclin group is comprised of four true members in Arabidopsis thaliana.B1‐type cyclins have a tissue‐specific, yet overlapping, role in plant development.CYCB1;2 is the most central B1‐type cyclin.Microtubule organization is regulated by B1‐type cyclins and likely achieved by phosphorylation of microtubule‐associated proteins. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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