| Autor: |
Trang Le, Ngoc Thuy, Nguyen, Ngoc Hoi, Hoang, Minh Chau, Khoa Nguyen, Cuu, Hai Nguyen, Dai, Tran, Dieu Linh |
| Předmět: |
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| Zdroj: |
Journal of Bioactive & Compatible Polymers; Jan2022, Vol. 37 Issue 1, p3-16, 14p |
| Abstrakt: |
Despite the wide-spectrum and effective anti-cancer activity of paclitaxel (PTX), their low solubility and side effects are the main challenges in their clinical application. In this study, a model paclitaxel-encapsulated nanoliposome (NLips-PTX) carrier was synthesized to enhance PTX solubility and increase its passive accumulation at the tumor site. Soy lecithin and cholesterol at a 9:1 ratio were used to prepare the nano-sized liposomes through the thin-film hydration followed by extrusion technique. The prepared spherical NLips-PTX liposomes with an average size of about 150 nm and high uniformity were characterized by DLS and TEM. PTX load efficiency of NLips was determined at about 85% by HPLC. NLips-PTX also showed a therapeutic effect toward breast cancer cells (MCF-7) in a dose- and time-dependent manner via in vitro cellular uptake and a cytotoxicity study. This research indicates that extrusion is a simple and convenient method for nano-sizing and homogenising liposome suspension for potentially effective delivery of drug to target tumor sites. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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