Genetic Diversity of Black Turmeric (Curcuma caesia Roxb.) Genotypes Identified by SSR Markers.

Autor: KUMAR, A. B. MOHAN, VASUNDHARA, M., SATHYANARAYANA, B. N., SHYAMALAMMA, S., DORESWAMY, C., ANIL, VEENA S.
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Zdroj: Mysore Journal of Agricultural Sciences; Oct-Dec2021, Vol. 55 Issue 4, p197-213, 17p
Abstrakt: Black turmeric is widely distributed in Indian subcontinent and having high economic importance due to varied medicinal properties. Presently the species is considered as critically endangered and mainly propagated vegetatively through underground rhizomes. Urgent need is there to conserve this rare and unconventional medicinally important plant. Genetic diversity studies in C. caesia Roxb. still in primitive stage for selecting the better performing accessions. SSR finger print for 33 black turmeric accessions by using sixteen primers revealed a total of 77 scorable bands. Among which 43 were polymorphic with an average of 2.68 per primer. SSR-02 and SSR 07 primers produced highest polymorphism (100%), followed by SSR-01, SSR-03, SSR-04, SSR-05 (75.00%). Whereas, SSR 10 produced least polymorphism (37.50%) and SSR 13, SSR 23 had showed no polymorphism. Overall primers exhibited 55.84 per cent polymorphism and the PIC value ranged between 0.255 to 0.480, which is the range to predict moderate diversity among the population. All genotypes were grouped in to three genetically diverse clusters and nine subgroups. Dendrogram depicts majority of the genotypes intermingled with each other in different clusters irrespective of their geographical affiliations. Twelve sets of genotypes had showed 100 per cent genetic similarity, while fourteen sets had least (10%). The observed intraspecific variation may be associated with cultivation and targeted genotype selection of desirable characters in C. caesia as the species is economically important. These results are the first step towards a better knowledge of the germplasm available in Indian subcontinent and can help to guide conservation of the species. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index