| Autor: |
BANDAKKANAVAR, MAHALINGAPPA, RAMAPPA, H. K., NAGARAJU, N., PRAMEELA, H. A., BYREGOWDA, M., SANJAY, M. T. |
| Předmět: |
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| Zdroj: |
Mysore Journal of Agricultural Sciences; Oct-Dec2021, Vol. 55 Issue 4, p189-196, 8p |
| Abstrakt: |
Amplified 1.8 kb and 1.2 kb fragment corresponding to the 16S rDNA and intergenic transcribed spacer region of the phytoplasma infecting pigeonpea, brinjal, sesamum, chickpea, sunhemp, buckwheat, datura and parthenium tissue using phytoplasma specific universal primers P1/P7 and R16F2n/R16FR2. Phylogenetic analysis of 16S rDNA gene of phytoplasma revealed that, Daturastramonium phytoplasma Shivamogga, pigeonpea phyllody GKVK (Accession No. MW559977), chickpea phyllody phytoplasma Doddaballapur (Accession No. MW583034) sunhemp phyllody GKVK (Accession No. MW543302) and buckwheat phyllody GKVK (Accession No. MW559978) were clustered with periwinkle little leaf phytoplasma (Accession No. AF228053.1) and shared nucleotide identity of 62.1 to 85.5 per cent. Similarly, sesamum phyllody GKVK (Accession No. MW559980) and parthenium phyllody GKVK (Accession No. MW559981) with 'Setaria verticillata' little leaf phytoplasma SVLL-GOK1 (Accession No.MW077129.1) with sequence similarity of 88.4 to 96.1 per cent, whereas, brinjal little leaf phytoplasma GKVK (Accession No.MW547094) clustered with Iranian safflower phyllody phytoplasma (Accession No. EF534205.1) and shared 92.4 per cent nucleotide identity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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