| Autor: |
Brozmanová, Jela, Vlčková, Viera, Chovanec, Miroslav |
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| Zdroj: |
Current Genetics; Nov2004, Vol. 46 Issue 6, p317-330, 14p |
| Abstrakt: |
DNA-damaging agents constantly challenge cellular DNA; and efficient DNA repair is therefore essential to maintain genome stability and cell viability. Several DNA repair mechanisms have evolved and these have been shown to be highly conserved from bacteria to man. DNA repair studies were originally initiated in very simple organisms such asEscherichia coliandSaccharomyces cerevisiae, bacteria being the best understood organism to date. As a consequence, bacterial DNA repair genes encoding proteins with well characterized functions have been transferred into higher organisms in order to increase repair capacity, or to complement repair defects, in heterologous cells. While indicating the contribution of these repair functions to protection against the genotoxic effects of DNA-damaging agents, heterologous expression studies also highlighted the role of the DNA lesions that are substrates for such processes. In addition, bacterial DNA repair-like functions could be identified in higher organisms using this approach. We heterologously expressed three well characterizedE. colirepair genes inS. cerevisiaecells of different genetic backgrounds: (1) theadagene encodingO6-methylguanine DNA-methyltransferase, a protein involved in the repair of alkylation damage to DNA, (2) therecAgene encoding the main recombinase inE. coliand (3) thenthgene, the product of which (endonuclease III) is responsible for the repair of oxidative base damage. Here, we summarize our results and indicate the possible implications they have for a better understanding of particular DNA repair processes inS. cerevisiae. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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