| Abstrakt: |
Plant phenolics are the largest group of secondary metabolites found in higher plants, which have a myriad of therapeutic properties, deployed as a remedy for treating many serious ailments. Phenolics are generally relevant in food, chemical, agriculture, cosmetic and pharmaceutical industries. Recently, the demand for plant derivatives is colossal; thus, reviving many protocols for extraction, isolation, elucidation of phytoconstituents of plants was intensified. There are numerous chromatographic and analytical techniques involved in the identification of specific bioactive compounds. In the current study, phenolics were extracted from the methanolic leaf extract of Costus pictus. Costus pictus is a rhizomatous perennial medicinal herb recognized as an insulin plant that has a place in the Costaceae family. The leaves are consumed as a dietary munching agent as it can reduce blood glucose level. Phenols were identified from the leaf extract of C. pictus by extraction of phytoconstituents from the leaf; it was done first by extracting with methanol solvent utilizing a Soxhlet apparatus. The preliminary phytochemical screening uncovers the presence of numerous phytoconstituents; later phytoconstituents were separated by eluting with organic solvent through column chromatography by petroleum ether and ethyl acetate as a descending and ascending order (9:1 to 1:9, V/V). All the column fractions were accumulated independently and eluted on thin-layer chromatography (TLC) plates for TLC analysis. The high-performance thin-layer chromatography analysis reveals the presence of phytoconstituents eluted on pre-coated TLC plates along with the standard compounds and distinguished by their RF values. The compounds with the same RF values were separated from preparative TLC plates, pooled together and characterized by high-performance liquid chromatography, diode array detector and tandem mass spectrometry (MS/MS). The three phenolic compounds, m/z 701 oleuropein glucoside, m/z 475 isorhamnetin-3-O-glucoside and m/z 179 caffeic acid, with corresponding daughter ions were identified. [ABSTRACT FROM AUTHOR] |
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