| Autor: |
Duarte-Neto, Amaro Nunes, Silva, Luiz Fernando Ferraz da, Monteiro, Renata Aparecida de Almeida, Filho, Jair Theodoro, Leite, Thabata Larissa Luciano Ferreira, Moura, Catia Sales de, Gomes-Gouvêa, Michele Soares, Pinho, João Renato Rebellho, Kanamura, Cristina Takami, Oliveria, Ellen Pierre de, Bispo, Kely Cristina Soares, Arruda, Cássia, Santos, Aline Brito dos, Aquino, Flavia Cristina Gonçalves, Caldini, Elia Garcia, Mauad, Thais, Saldiva, Paulo Hilário Nascimento, Dolhnikoff, Marisa |
| Předmět: |
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| Zdroj: |
Clinical Infectious Diseases; 2021 Supplement, Vol. 73, pS442-S453, 12p |
| Abstrakt: |
Background Minimally invasive autopsies, also known as minimally invasive tissue sampling (MITS), have proven to be an alternative to complete diagnostic autopsies (CDAs) in places or situations where this procedure cannot be performed. During the coronavirus disease 2019 (COVID-19) pandemic, CDAs were suspended by March 2020 in Brazil to reduce biohazard. To contribute to the understanding of COVID-19 pathology, we have conducted ultrasound (US)–guided MITS as a strategy. Methods This case series study includes 80 autopsies performed in patients with COVID-19 confirmed by laboratorial tests. Different organs were sampled using a standardized MITS protocol. Tissues were submitted to histopathological analysis as well as immunohistochemical and molecular analysis and electron microscopy in selected cases. Results US-guided MITS proved to be a safe and highly accurate procedure; none of the personnel were infected, and accuracy ranged from 69.1% for kidney, up to 90.1% for lungs, and reaching 98.7% and 97.5% for liver and heart, respectively. US-guided MITS provided a systemic view of the disease, describing the most common pathological findings and identifying viral and other infectious agents using ancillary techniques, and also allowed COVID-19 diagnosis confirmation in 5% of the cases that were negative in premortem and postmortem nasopharyngeal/oropharyngeal swab real-time reverse-transcription polymerase chain reaction. Conclusions Our data showed that US-guided MITS has the capacity similar to CDA not only to identify but also to characterize emergent diseases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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