| Autor: |
Juanjuan Wang, Xin Liu, Jing Yang, Hanxing Guo, Jingjing Li, Lihui Huo, Haitao Zhao, Xiaowei Wang, Xingrong Yan, Baoguo Li, Yu sun |
| Předmět: |
|
| Zdroj: |
Journal of Medical Primatology; Dec2021, Vol. 50 Issue 6, p323-331, 9p |
| Abstrakt: |
Background: Golden snub-nosed monkey (Rhinopithecus roxellana) is an endangered primate species, whose molecular material for conservation purposes has not yet been maintained. Although small-molecule compounds (SMCs) have been reported to improve induced pluripotent stem cells (iPSCs), their efficiency in the interspecies-transferred nucleus is still unknown. Methods: We thus used the fibroblasts from the golden snub-nosed monkey treated with SMC as donor cells, injected into the enucleated oocytes of goats, to test such efficiency. Gene expression profiles in the cell-constructed embryos with and without SMCs were compared by qPCR. Results: The results show that cell morphology undergoes remarkable changes (volume is smaller than normal cells, and many black spots in the cytoplasm were found); pluripotent genes (Oct4, Sox2, and Nanog) significantly increased with SMC treatment. Conclusions: This study demonstrates that SMCs alter the properties of donor cells and promote the expression of pluripotent genes in hybrid embryos. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
