| Autor: |
Pozdina, V. A., Zvedeninova, U. V., Ulitko, M. V., Danilova, I. G., Abidov, M. T. |
| Zdroj: |
Cell & Tissue Biology; Nov2021, Vol. 15 Issue 6, p594-603, 10p |
| Abstrakt: |
Morphometric and immunophenotypic characteristics of rat bone marrow macrophages isolated from intact animals and stimulated with sodium aminodihydrophthalazinedione (ADPN) under cultivation for 24, 48, and 72 h were studied. The following morphometric parameters were determined: the cell area, the area of the cytoplasm and nuclei, as well as the nuclear-cytoplasmic ratio (NCR). The proliferative activity was evaluated on the basis of the presence of the Ki-67 marker. The phenotype of macrophages was determined by the markers CD163 (M2 phenotype) and F4/80 (M1 phenotype) present on the cell surface. In addition, the content of the TGF-β growth factor in the macrophage cytoplasm was evaluated. The stimulation of macrophages with ADPN at a dose of 50 μg/mL promotes an increase in the number of CD163+ cells and the content of this marker with increasing cultivation time. On the contrary, the action of 100 μg/mL ADPN leads to an increase in the proportion of F4/80+ cells in the culture and to the growth in the content of this marker with increasing cultivation time. The accumulation of TGF-β occurs for 48 h of cell culturing in the presence of 50 μg/mL ADPN, and its increased content is retained for 72 h. ADPN at the dose of 100 μg/mL enhances the formation of TGF-β to 48 h and inhibits it to 72 h of cell culturing with the substance. The maturation of macrophages is accelerated when ADPN is added to the culture of monocytes, and the substance also has a pronounced dose-dependent effect on BM macrophages. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink