| Autor: |
Pereira, Wander Lopes, de Oliveira, Tânia Toledo, Kanashiro, Milton Masahiko, Filardi, Marcelo Augusto, da Costa, Marcelo Rocha, da Costa, Liovando Marciano |
| Předmět: |
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| Zdroj: |
Natural Product Research; Dec 2021, Vol. 35 Issue 23, p5554-5558, 5p |
| Abstrakt: |
The present study investigated the possibility of apoptosis-inducing activity in human leukemia U-937 and THP-1 cells by the flavonoid morin. The treatments were evaluated by using the MTT and LDH assays; analysis of mitochondrial membrane potential (ΔΨm) was evaluated by flow cytometry, cell death by apoptosis was confirmed by fluorescence microscopy and by assessing the activity of caspases-3 and −6. The data indicated that the flavonoid morin has promoted a decrease in cell viability in a concentration-dependent way for both of the cancerous cell lines. An increase in the percentage of cell death caused by apoptosis was associated to a potential alteration in the mitochondrial membrane (ΔΨm) suggesting the involvement of cell death in intrinsic apoptotic pathways. Activation of caspases-3 and −6 confirmed the presence of apoptotic activity from morin. The results reinforce the antileukemic potential of flavonol morin. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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