Sinoacutine inhibits inflammatory responses to attenuates acute lung injury by regulating NF-κB and JNK signaling pathways.
Autor: | Zhao, Yuancui, Cui, Lili, Yang, Xing Xin, Sun, Xingqian, Liu, Yunkuan, Yang, Zixian, Zhu, Liyuan, Peng, Chaorui, Li, Danye, Cai, Junfei, Ma, Yunshu |
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Předmět: |
THERAPEUTIC use of alkaloids
THERAPEUTIC use of plant extracts LUNG injuries LIPOPOLYSACCHARIDES BIOLOGICAL models CYTOKINES INTERLEUKINS ANTI-inflammatory agents ANIMAL experimentation WESTERN immunoblotting INFLAMMATION MACROPHAGES CELLULAR signal transduction GENE expression DNA-binding proteins TRANSFERASES RESEARCH funding TUMOR necrosis factors POLYMERASE chain reaction NITRIC oxide ACUTE diseases MICE |
Zdroj: | BMC Complementary Medicine & Therapies; 11/20/2021, Vol. 21 Issue 1, p1-10, 10p, 1 Diagram, 2 Charts, 3 Graphs |
Abstrakt: | Background: Stephania yunnanensis H. S. Lo is widely used as an antipyretic, analgesic and anti-inflammatory herbal medicine in SouthWest China. In this study, we investigated the anti-inflammatory activity and mechanism of sinoacutine (sino), one of the primary components extracted from this plant. Methods: A RAW264.7 cell model was established using lipopolysaccharide (LPS) induced for estimation of cytokines in vitro, qPCR was used to estimate gene expression, western blot analysis was used to estimate protein level and investigate the regulation of NF- κB, JNK and MAPK signal pathway. In addition, an acute lung injury model was established to determine lung index and levels of influencing factors. Results: Using the RAW264.7 model, we found that sino reduced levels of nitric oxide (NO), tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and prostaglandin E |
Databáze: | Complementary Index |
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