| Autor: |
Lengliz, S., Benlabidi, S., Raddaoui, A., Cheriet, S., Ben Chehida, N., Najar, T., Abbassi, M.S. |
| Předmět: |
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| Zdroj: |
Letters in Applied Microbiology; Dec2021, Vol. 73 Issue 6, p708-717, 10p |
| Abstrakt: |
We aimed to study the antibiotic susceptibility and possible occurrence of extended‐spectrum beta‐lactamases (ESBL)/carbapenemase‐producing Escherichia coli isolates collected from rabbits in Tunisia. In all, 35 faecal samples from healthy rabbits were collected from one farm and E. coli were isolated from three media: antibiotic‐free TBX agar, TBX+2 mg l−1 cefotaxime and TBX+1 mg l−1 imipenem. In total, 39 E. coli isolates were recovered; the majority showed resistance to at least one antibiotic and none was ESBL producer. Carbapenem resistance was detected in 16 isolates from either selective or un‐selective media. Phenotypic methods used to detect carbapenemase production showed two positive isolates by Modified Hodge Test, six metallo‐carbapenemase producers (Imipenem disc+EDTA) and all were temocillin resistant (possible OXA‐48 carbapenemase). blaVIM and blaIMP type genes were detected in two and one isolates, respectively; one of them harboured both genes. Isolates contained common genes encoding resistance to sulphonamides (sul1, sul2), tetracycline (tetA, tetB, tetC) and fluoroquinolones (qnrS, aac(6′)‐Ib‐cr). Class 1 and 2 integrons were detected in five and four isolates, respectively. These findings highlight the importance of rabbit production as reservoir of carbapenem‐resistant E. coli and argument the first report of blaVIM and blaIMP genes in livestock in Tunisia. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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