| Autor: |
Ren, Sida, Zhang, Xi, Li, Zhijie, Jian, Xiaoxia, Zhao, Junjian, Song, Yan-Yan |
| Předmět: |
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| Zdroj: |
Microchimica Acta; Nov2021, Vol. 188 Issue 11, p1-11, 11p |
| Abstrakt: |
Pathogenic bacteria can cause the outbreaks of disease and threaten human health, which stimulates the development of advanced detection techniques. Herein, a specific and sensitive electrochemical biosensor for Gram-negative bacteria was established based on the conductive polymer with artificial muscle properties. The effective recognition was achieved through the specific carbohydrate-carbohydrate interaction between gluconamide and lipopolysaccharide. The application of impulse voltage enhances the efficiency of recognition and shortens the detection time through the temporary deformation of the electrode surface, with a limit of detection (LOD) of 1 × 100 CFU/mL and a linear range of 1 × 100 − 1 × 106 CFU/mL for Escherichia coli (E. coli). In addition to the merits of low cost, high efficiency, and rapidity, the developed label-free electrochemical biosensor can also be applicable for other Gram-negative bacteria, owning promising potential in the application of portable devices and paving a potential way for the construction of electrochemical biosensors. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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