| Autor: |
Alrefaei, Abdulwahed Fahad, Alrefaei, Abdulmajeed F. |
| Předmět: |
|
| Zdroj: |
Umm Al-Qura University Journal of Applied Sciences (Association of Arab Universities); Jul2021, Vol. 7 Issue 1, p23-26, 4p |
| Abstrakt: |
The parasite Trichomonas gallinae causes trichomonosis in avian and has been reported on several birds, meaning a worldwide prevalence. Infected birds usually become malnourished, because huge necrotic and inflammatory lesions in the mouth and crop prohibit them from swallowing water and food. T. gallinae threatens bird populations, including endangered birds, so it is important to improve methods for detecting infected birds. Currently, the used method to identify T. gallinae depend on direct observation of the parasites via light microscopy, but it has failed to detect dangerous strains. Here, we describe a detailed molecular method that will enable researchers and veterinarians to detect and diagnose avian trichomonosis. Polymerase chain reaction (PCR) can be an extremely sensitive and specific tool for identifying and studying T. gallinae genetics. In this efficient method, samples from birds are cultured and amplified via PCR and then the products are sequenced to allow genetic characterization of T. gallinae and construction of its phylogenetic tree. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
