Improved use of organic phosphate by Skeletonema costatum through regulation of Zn2 + concentrations.

Autor: Yanjun Shi, Hanhua Hu, Runyu Ma, Wei Cong, Zhaoling Cai
Předmět:
Zdroj: Biotechnology Letters; May2004, Vol. 26 Issue 9, p747-751, 5p
Abstrakt: The maximum growth rate (1.4–2 × 105 cells ml-1 d-1), cell final yields (2.6–5.2 × 105 cells ml-1) and extracellular alkaline phosphatase activity (2.4–10.6 μg phosphate released ml-1 h-1) of the red tide alga, Skeletonema costatum, increased when Zn2+ was increased from 0 to 24 pm, but decreased with 66 pm Zn2+ in growth medium with glycerophosphate as the sole phosphorus source. Extracellular carbonic anhydrase activity and the affinity for HCO3- and CO2 uptake increased when Zn2+ was increased from 0 to 12 pm, but then decreased at higher concentrations. The results suggested that utilization of organic phosphate required more Zn2+ than the uptake of inorganic carbon did, while utilization of dissolved inorganic carbon by Skeletonema costatum was very sensitive to Zn2+ concentration variations. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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