| Autor: |
Beyi, Ashenafi Feyisa, Brito-Goulart, Debora, Hawbecker, Tyler, Ruddell, Brandon, Hassall, Alan, Dewell, Renee, Dewell, Grant, Sahin, Orhan, Zhang, Qijing, Plummer, Paul J. |
| Předmět: |
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| Zdroj: |
Microorganisms; Oct2021, Vol. 9 Issue 10, p2162-2162, 1p |
| Abstrakt: |
Enrofloxacin is a fluoroquinolone drug used to prevent and control bovine respiratory disease (BRD) complex in multiple or single doses, ranging from 7.5 to 12.5 mg/kg body weight. Here, we examined the effects of high and low doses of a single subcutaneously injected enrofloxacin on gut microbiota and resistome in calves. Thirty-five calves sourced for this study were divided into five groups: control (n = 7), two low dose groups (n = 14, 7.5 mg/kg), and two high dose groups (n = 14, 12.5 mg/kg). One group in the low and high dose groups was challenged with Mannheimia haemolytica to induce BRD. Both alpha and beta diversities were significantly different between pre- and post-treatment microbial communities (q < 0.05). The high dose caused a shift in a larger number of genera than the low dose. Using metagenomic ProxiMeta Hi-C, 32 unique antimicrobial resistance genes (ARGs) conferring resistance to six antibiotic classes were detected with their reservoirs, and the high dose favored clonal expansion of ARG-carrying bacterial hosts. In conclusion, enrofloxacin treatment can alter fecal microbiota and resistome irrespective of its dose. Hi-C sequencing provides significant benefits for unlocking new insights into the ARG ecology of complex samples; however, limitations in sample size and sequencing depth suggest that further work is required to validate the findings. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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